Comparison of the effects of desflurane and sevoflurane on the expression of platelet surface glycoproteins in unstimulated and adenosine diphosphate-induced platelets in vitro{star, open}

Study Objective: To compare the effects of one minimum alveolar concentration (MAC) desflurane and sevoflurane on the expression of CD42b (glycoprotein [GP] Ib), CD41 (GPIIb), CD61 (GPIIIa), CD62P (P-selectin), and CD63 in both unstimulated and adenosine diphosphate (ADP)-stimulated platelets in vitro. Setting: University laboratory. Subjects: 15 healthy volunteers. Interventions: Platelet-rich plasma was obtained and divided into three groups: platelet-rich plasma exposed to air (group 1); air plus one MAC desflurane (6% vol; group 2), and air plus one MAC sevoflurane (2% vol; group 3), for 40 minutes. Percentage of antigen-positive cells (%⁺) mean channel fluorescence (MCF^Σ), and index of platelet activation for positive platelets (IPA⁺) as expression markers for GPIb, GPIIb, GPIIIa, P-selectin, and CD63, were measured. Measurements and Main Results: In unstimulated platelets, expression markers for GPIIb and GPIIIa were significantly lower in groups 2 and 3 than group 1 (P < 0.001). P-selectin expression markers were significantly higher in group 2 than in group 1 or group 3 (P < 0.016). CD63 expression markers were significantly lower in group 3 than group 1 (P < 0.016). In ADP-stimulated platelets, expression markers for all glycoproteins were significantly higher in all groups. Conclusion: Neither one MAC desflurane nor sevoflurane showed any significant change in ADP-stimulated platelets compared with the control group. © 2007 Elsevier Inc. All rights reserved.