Epigallocatechin-3-gallate and resveratrol attenuate hydrogen peroxide induced damage in neuronal cells

PURPOSE: The purpose of this study is to investigate how the antioxidants resveratrol and epigallocatechin-3-gallate (EGCG) protect SH-SY5Y cells against damage caused by hydrogen peroxide (H2O2). METHODS: SH-SY5Y cells were pretreated with EGCG and resveratrol at concentrations of 0.1 μM, 1 μM, and 10 μM, individually and in various combinations. Cells were exposed to 250 μM H2O2 for 1-hour following a 24-hour pretreatment. The effects of EGCG and resveratrol on cellular survival against hydrogen peroxide toxicity were evaluated with the MTS. Caspase 3 levels were measured with caspase 3 ELISA test for evaluating survival. The clonogenic test was utilized to assess the colony forming capacity. RESULTS: The MTS test revealed that pretreatment of SH-SY5Y cells for 24 hours with EGCG and Resveratrol enhanced cellular survival against hydrogen peroxide damage at all dosages (p < 0.005). The caspase 3 ELISA test revealed that EGCG and resveratrol signifi cantly decreased caspase 3 levels and improved cellular survival via the caspase 3 pathway (p < 0.005). The clonogenic test fi ndings show that resveratrol and EGCG statistically boost SH-SY5Y cells’ potential to form colonies (p<0.005). CONCLUSIONS: By reducing caspase 3 levels in exposure to hydrogen peroxide damage, EGCG and resveratrol promote cellular viability (Tab. 1, Fig. 3, Ref. 37)