Production of Collagen Modified PDLA Biofilms

Enhancing the biological functionality of polymeric biomaterials, particularly cell adhesion, proliferation, and biocompatibility, is essential for developing functional applications in tissue engineering. In this work, poly(D-lactic acid) (PDLA) was covalently grafted with collagen (PDLA-g-Coll) to overcome PDLA's inherent limitations in cellular interactions. The hybrid films were fabricated via solvent casting and subjected to comprehensive biological evaluation. In vitro experiments using L929 mouse fibroblast cells, demonstrated a marked enhancement in cell attachment and proliferation on the PDLA-g-Coll films compared to unmodified PDLA and pure collagen controls, as confirmed by MTT assays. These improvements are attributed to the increased surface hydrophilicity conferred by the grafted collagen chains, a finding corroborated by contact angle measurements. The favorable surface morphology of the PDLA-g-Coll films further supported cell proliferation. Collectively, these results highlight the synergistic integration of PDLA's mechanical strength and biodegradability with collagen's bioactivity, yielding a composite material with significant potential for application in tissue engineering.